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casein solution  (Thermo Fisher)


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    Thermo Fisher casein solution
    Casein Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/casein+solution/Casein+Blocking+Buffer%2C+3%25+in+PBS/10__1080_slash_0886022x__2026__2676320-67-83-87
    Average 95 stars, based on 1 article reviews
    casein solution - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: PAT-on-a-chip: Miniaturization of analytical assays towards data-driven bioprocess development and optimization.
    Article Snippet: .. The workflow for the IgG titer and CHO HCP assays was as follows: i) blocking of the bead-packed channels with 50 μL of casein solution (1 % w/v, Thermo Fisher Scientific) at 5 μL/min; ii) flowing of 100 μL sample previously pre-incubated with HRP-conjugated detection antibodies at optimized concentrations (1h or 2h off-chip incubation for the IgG and CHO HCP assay, respectively) at 3 μL/min; iii) washing twice with 100 μL of 1 × PBS/0.05 % Tween 20 at 10 μL/min; and iv) flowing of 50 μL TMB blotting solution at 5 μL/min. .. The workflow for the glycosylation assay was as follows: i) blocking of the bead-packed channel with 50 μL of 1 % BSA solution (Blocker BSA, Thermo Fisher Scientific) at 5 μL/min; ii) flowing of 100 μL sample at 10 μL/min; iii) washing with 50 μL of PBS at 10 μL/min; iv) flowing of 100 μL of biotinylated lectin marker solution at optimized concentrations at 10 μL/min; v) washing with 100 μL of previously optimized washing buffer at 10 μL/min; vi) flowing of 100 μL of 125 ng/mL HRPconjugated streptavidin (Thermo Fisher Scientific) at 10 μL/min; vii) washing twice with 100 μL of 1 × PBS/0.05 % Tween 20 at 10 μL/min; and vii) flowing of 50 μL TMB blotting solution at 5 μL/min.

    Blocking Assay:

    Article Title: PAT-on-a-chip: Miniaturization of analytical assays towards data-driven bioprocess development and optimization.
    Article Snippet: .. The workflow for the IgG titer and CHO HCP assays was as follows: i) blocking of the bead-packed channels with 50 μL of casein solution (1 % w/v, Thermo Fisher Scientific) at 5 μL/min; ii) flowing of 100 μL sample previously pre-incubated with HRP-conjugated detection antibodies at optimized concentrations (1h or 2h off-chip incubation for the IgG and CHO HCP assay, respectively) at 3 μL/min; iii) washing twice with 100 μL of 1 × PBS/0.05 % Tween 20 at 10 μL/min; and iv) flowing of 50 μL TMB blotting solution at 5 μL/min. .. The workflow for the glycosylation assay was as follows: i) blocking of the bead-packed channel with 50 μL of 1 % BSA solution (Blocker BSA, Thermo Fisher Scientific) at 5 μL/min; ii) flowing of 100 μL sample at 10 μL/min; iii) washing with 50 μL of PBS at 10 μL/min; iv) flowing of 100 μL of biotinylated lectin marker solution at optimized concentrations at 10 μL/min; v) washing with 100 μL of previously optimized washing buffer at 10 μL/min; vi) flowing of 100 μL of 125 ng/mL HRPconjugated streptavidin (Thermo Fisher Scientific) at 10 μL/min; vii) washing twice with 100 μL of 1 × PBS/0.05 % Tween 20 at 10 μL/min; and vii) flowing of 50 μL TMB blotting solution at 5 μL/min.

    Incubation:

    Article Title: PAT-on-a-chip: Miniaturization of analytical assays towards data-driven bioprocess development and optimization.
    Article Snippet: .. The workflow for the IgG titer and CHO HCP assays was as follows: i) blocking of the bead-packed channels with 50 μL of casein solution (1 % w/v, Thermo Fisher Scientific) at 5 μL/min; ii) flowing of 100 μL sample previously pre-incubated with HRP-conjugated detection antibodies at optimized concentrations (1h or 2h off-chip incubation for the IgG and CHO HCP assay, respectively) at 3 μL/min; iii) washing twice with 100 μL of 1 × PBS/0.05 % Tween 20 at 10 μL/min; and iv) flowing of 50 μL TMB blotting solution at 5 μL/min. .. The workflow for the glycosylation assay was as follows: i) blocking of the bead-packed channel with 50 μL of 1 % BSA solution (Blocker BSA, Thermo Fisher Scientific) at 5 μL/min; ii) flowing of 100 μL sample at 10 μL/min; iii) washing with 50 μL of PBS at 10 μL/min; iv) flowing of 100 μL of biotinylated lectin marker solution at optimized concentrations at 10 μL/min; v) washing with 100 μL of previously optimized washing buffer at 10 μL/min; vi) flowing of 100 μL of 125 ng/mL HRPconjugated streptavidin (Thermo Fisher Scientific) at 10 μL/min; vii) washing twice with 100 μL of 1 × PBS/0.05 % Tween 20 at 10 μL/min; and vii) flowing of 50 μL TMB blotting solution at 5 μL/min.

    Article Title: Exertional heat stroke-related acute kidney injury: novel animal and cellular models
    Article Snippet: Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, dojindo, CK04, 1:10) according to the manufacturer’s instructions. .. after the cells were lysed using radio Immunoprecipitation assay (rIPa, Servicebio, G2002, 1:2) buffer containing phenylmethylsulfonyl fluoride (PMSF, Solarbio, P0100, 1:100), the supernatant was collected by centrifugation, and the protein concentration was assessed using a bicinchoninic acid assay (bCa) protein assay kit (thermo, 23227). a total of 20 μg of protein was loaded per lane onto a 12% sodium dodecyl sulfate-polyacrylamide gel (SdS-PaGE, bIo-rad, 4561043) and subsequently transferred to a nitrocellulose (NC) membrane. the membrane was blocked at room temperature with a 1× casein solution (50 μL, thermo, 37582) for 2 h and then incubated overnight at 4 °C with the following antibodies: neutrophil gelatinase-associated lipocalin (NGaL, 10 mL, abcam, ab26426, 1:1000) and β-tubulin (10 mL, Proteintech, 66240-1, 1:20, 000). the membrane was then incubated with the corresponding secondary antibody (10 mL, beyotime, a0208, 1:2000) at room temperature for 2 h, followed by three washes with tris buffered saline with tween (tbSt, Solarbio, t1081, 1:10). .. Signals were detected using the ChemidocTM touch Imaging System (bio-rad, Hercules, Ca, uSa) with enhanced chemiluminescence (ECL, 2 mL, amersham biosciences, GErPN2235, 1:1), and the data were analyzed using ImageJ software (version 1.8.0, Md, uSa).

    Article Title: Alterations in protein N -glycosylation confer vanadate resistance in Ogataea polymorpha mutants defective in phosphomannosylation
    Article Snippet: .. The unbound antibody was removed by incubation of membrane in TBS 3 times for 5 min. Then the membranes were incubated in the casein solution supplemented with the goat anti-rabbit IgG horseradish peroxidase conjugate (ThermoFisher Scientific, cat. #31460) at a dilution of 1:10,000. ..

    Radio Immunoprecipitation:

    Article Title: Exertional heat stroke-related acute kidney injury: novel animal and cellular models
    Article Snippet: Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, dojindo, CK04, 1:10) according to the manufacturer’s instructions. .. after the cells were lysed using radio Immunoprecipitation assay (rIPa, Servicebio, G2002, 1:2) buffer containing phenylmethylsulfonyl fluoride (PMSF, Solarbio, P0100, 1:100), the supernatant was collected by centrifugation, and the protein concentration was assessed using a bicinchoninic acid assay (bCa) protein assay kit (thermo, 23227). a total of 20 μg of protein was loaded per lane onto a 12% sodium dodecyl sulfate-polyacrylamide gel (SdS-PaGE, bIo-rad, 4561043) and subsequently transferred to a nitrocellulose (NC) membrane. the membrane was blocked at room temperature with a 1× casein solution (50 μL, thermo, 37582) for 2 h and then incubated overnight at 4 °C with the following antibodies: neutrophil gelatinase-associated lipocalin (NGaL, 10 mL, abcam, ab26426, 1:1000) and β-tubulin (10 mL, Proteintech, 66240-1, 1:20, 000). the membrane was then incubated with the corresponding secondary antibody (10 mL, beyotime, a0208, 1:2000) at room temperature for 2 h, followed by three washes with tris buffered saline with tween (tbSt, Solarbio, t1081, 1:10). .. Signals were detected using the ChemidocTM touch Imaging System (bio-rad, Hercules, Ca, uSa) with enhanced chemiluminescence (ECL, 2 mL, amersham biosciences, GErPN2235, 1:1), and the data were analyzed using ImageJ software (version 1.8.0, Md, uSa).

    Centrifugation:

    Article Title: Exertional heat stroke-related acute kidney injury: novel animal and cellular models
    Article Snippet: Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, dojindo, CK04, 1:10) according to the manufacturer’s instructions. .. after the cells were lysed using radio Immunoprecipitation assay (rIPa, Servicebio, G2002, 1:2) buffer containing phenylmethylsulfonyl fluoride (PMSF, Solarbio, P0100, 1:100), the supernatant was collected by centrifugation, and the protein concentration was assessed using a bicinchoninic acid assay (bCa) protein assay kit (thermo, 23227). a total of 20 μg of protein was loaded per lane onto a 12% sodium dodecyl sulfate-polyacrylamide gel (SdS-PaGE, bIo-rad, 4561043) and subsequently transferred to a nitrocellulose (NC) membrane. the membrane was blocked at room temperature with a 1× casein solution (50 μL, thermo, 37582) for 2 h and then incubated overnight at 4 °C with the following antibodies: neutrophil gelatinase-associated lipocalin (NGaL, 10 mL, abcam, ab26426, 1:1000) and β-tubulin (10 mL, Proteintech, 66240-1, 1:20, 000). the membrane was then incubated with the corresponding secondary antibody (10 mL, beyotime, a0208, 1:2000) at room temperature for 2 h, followed by three washes with tris buffered saline with tween (tbSt, Solarbio, t1081, 1:10). .. Signals were detected using the ChemidocTM touch Imaging System (bio-rad, Hercules, Ca, uSa) with enhanced chemiluminescence (ECL, 2 mL, amersham biosciences, GErPN2235, 1:1), and the data were analyzed using ImageJ software (version 1.8.0, Md, uSa).

    Protein Concentration:

    Article Title: Exertional heat stroke-related acute kidney injury: novel animal and cellular models
    Article Snippet: Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, dojindo, CK04, 1:10) according to the manufacturer’s instructions. .. after the cells were lysed using radio Immunoprecipitation assay (rIPa, Servicebio, G2002, 1:2) buffer containing phenylmethylsulfonyl fluoride (PMSF, Solarbio, P0100, 1:100), the supernatant was collected by centrifugation, and the protein concentration was assessed using a bicinchoninic acid assay (bCa) protein assay kit (thermo, 23227). a total of 20 μg of protein was loaded per lane onto a 12% sodium dodecyl sulfate-polyacrylamide gel (SdS-PaGE, bIo-rad, 4561043) and subsequently transferred to a nitrocellulose (NC) membrane. the membrane was blocked at room temperature with a 1× casein solution (50 μL, thermo, 37582) for 2 h and then incubated overnight at 4 °C with the following antibodies: neutrophil gelatinase-associated lipocalin (NGaL, 10 mL, abcam, ab26426, 1:1000) and β-tubulin (10 mL, Proteintech, 66240-1, 1:20, 000). the membrane was then incubated with the corresponding secondary antibody (10 mL, beyotime, a0208, 1:2000) at room temperature for 2 h, followed by three washes with tris buffered saline with tween (tbSt, Solarbio, t1081, 1:10). .. Signals were detected using the ChemidocTM touch Imaging System (bio-rad, Hercules, Ca, uSa) with enhanced chemiluminescence (ECL, 2 mL, amersham biosciences, GErPN2235, 1:1), and the data were analyzed using ImageJ software (version 1.8.0, Md, uSa).

    Acid Assay:

    Article Title: Exertional heat stroke-related acute kidney injury: novel animal and cellular models
    Article Snippet: Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, dojindo, CK04, 1:10) according to the manufacturer’s instructions. .. after the cells were lysed using radio Immunoprecipitation assay (rIPa, Servicebio, G2002, 1:2) buffer containing phenylmethylsulfonyl fluoride (PMSF, Solarbio, P0100, 1:100), the supernatant was collected by centrifugation, and the protein concentration was assessed using a bicinchoninic acid assay (bCa) protein assay kit (thermo, 23227). a total of 20 μg of protein was loaded per lane onto a 12% sodium dodecyl sulfate-polyacrylamide gel (SdS-PaGE, bIo-rad, 4561043) and subsequently transferred to a nitrocellulose (NC) membrane. the membrane was blocked at room temperature with a 1× casein solution (50 μL, thermo, 37582) for 2 h and then incubated overnight at 4 °C with the following antibodies: neutrophil gelatinase-associated lipocalin (NGaL, 10 mL, abcam, ab26426, 1:1000) and β-tubulin (10 mL, Proteintech, 66240-1, 1:20, 000). the membrane was then incubated with the corresponding secondary antibody (10 mL, beyotime, a0208, 1:2000) at room temperature for 2 h, followed by three washes with tris buffered saline with tween (tbSt, Solarbio, t1081, 1:10). .. Signals were detected using the ChemidocTM touch Imaging System (bio-rad, Hercules, Ca, uSa) with enhanced chemiluminescence (ECL, 2 mL, amersham biosciences, GErPN2235, 1:1), and the data were analyzed using ImageJ software (version 1.8.0, Md, uSa).

    Bicinchoninic Acid Protein Assay:

    Article Title: Exertional heat stroke-related acute kidney injury: novel animal and cellular models
    Article Snippet: Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, dojindo, CK04, 1:10) according to the manufacturer’s instructions. .. after the cells were lysed using radio Immunoprecipitation assay (rIPa, Servicebio, G2002, 1:2) buffer containing phenylmethylsulfonyl fluoride (PMSF, Solarbio, P0100, 1:100), the supernatant was collected by centrifugation, and the protein concentration was assessed using a bicinchoninic acid assay (bCa) protein assay kit (thermo, 23227). a total of 20 μg of protein was loaded per lane onto a 12% sodium dodecyl sulfate-polyacrylamide gel (SdS-PaGE, bIo-rad, 4561043) and subsequently transferred to a nitrocellulose (NC) membrane. the membrane was blocked at room temperature with a 1× casein solution (50 μL, thermo, 37582) for 2 h and then incubated overnight at 4 °C with the following antibodies: neutrophil gelatinase-associated lipocalin (NGaL, 10 mL, abcam, ab26426, 1:1000) and β-tubulin (10 mL, Proteintech, 66240-1, 1:20, 000). the membrane was then incubated with the corresponding secondary antibody (10 mL, beyotime, a0208, 1:2000) at room temperature for 2 h, followed by three washes with tris buffered saline with tween (tbSt, Solarbio, t1081, 1:10). .. Signals were detected using the ChemidocTM touch Imaging System (bio-rad, Hercules, Ca, uSa) with enhanced chemiluminescence (ECL, 2 mL, amersham biosciences, GErPN2235, 1:1), and the data were analyzed using ImageJ software (version 1.8.0, Md, uSa).

    Membrane:

    Article Title: Exertional heat stroke-related acute kidney injury: novel animal and cellular models
    Article Snippet: Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, dojindo, CK04, 1:10) according to the manufacturer’s instructions. .. after the cells were lysed using radio Immunoprecipitation assay (rIPa, Servicebio, G2002, 1:2) buffer containing phenylmethylsulfonyl fluoride (PMSF, Solarbio, P0100, 1:100), the supernatant was collected by centrifugation, and the protein concentration was assessed using a bicinchoninic acid assay (bCa) protein assay kit (thermo, 23227). a total of 20 μg of protein was loaded per lane onto a 12% sodium dodecyl sulfate-polyacrylamide gel (SdS-PaGE, bIo-rad, 4561043) and subsequently transferred to a nitrocellulose (NC) membrane. the membrane was blocked at room temperature with a 1× casein solution (50 μL, thermo, 37582) for 2 h and then incubated overnight at 4 °C with the following antibodies: neutrophil gelatinase-associated lipocalin (NGaL, 10 mL, abcam, ab26426, 1:1000) and β-tubulin (10 mL, Proteintech, 66240-1, 1:20, 000). the membrane was then incubated with the corresponding secondary antibody (10 mL, beyotime, a0208, 1:2000) at room temperature for 2 h, followed by three washes with tris buffered saline with tween (tbSt, Solarbio, t1081, 1:10). .. Signals were detected using the ChemidocTM touch Imaging System (bio-rad, Hercules, Ca, uSa) with enhanced chemiluminescence (ECL, 2 mL, amersham biosciences, GErPN2235, 1:1), and the data were analyzed using ImageJ software (version 1.8.0, Md, uSa).

    Article Title: Alterations in protein N -glycosylation confer vanadate resistance in Ogataea polymorpha mutants defective in phosphomannosylation
    Article Snippet: .. The unbound antibody was removed by incubation of membrane in TBS 3 times for 5 min. Then the membranes were incubated in the casein solution supplemented with the goat anti-rabbit IgG horseradish peroxidase conjugate (ThermoFisher Scientific, cat. #31460) at a dilution of 1:10,000. ..

    Saline:

    Article Title: Exertional heat stroke-related acute kidney injury: novel animal and cellular models
    Article Snippet: Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, dojindo, CK04, 1:10) according to the manufacturer’s instructions. .. after the cells were lysed using radio Immunoprecipitation assay (rIPa, Servicebio, G2002, 1:2) buffer containing phenylmethylsulfonyl fluoride (PMSF, Solarbio, P0100, 1:100), the supernatant was collected by centrifugation, and the protein concentration was assessed using a bicinchoninic acid assay (bCa) protein assay kit (thermo, 23227). a total of 20 μg of protein was loaded per lane onto a 12% sodium dodecyl sulfate-polyacrylamide gel (SdS-PaGE, bIo-rad, 4561043) and subsequently transferred to a nitrocellulose (NC) membrane. the membrane was blocked at room temperature with a 1× casein solution (50 μL, thermo, 37582) for 2 h and then incubated overnight at 4 °C with the following antibodies: neutrophil gelatinase-associated lipocalin (NGaL, 10 mL, abcam, ab26426, 1:1000) and β-tubulin (10 mL, Proteintech, 66240-1, 1:20, 000). the membrane was then incubated with the corresponding secondary antibody (10 mL, beyotime, a0208, 1:2000) at room temperature for 2 h, followed by three washes with tris buffered saline with tween (tbSt, Solarbio, t1081, 1:10). .. Signals were detected using the ChemidocTM touch Imaging System (bio-rad, Hercules, Ca, uSa) with enhanced chemiluminescence (ECL, 2 mL, amersham biosciences, GErPN2235, 1:1), and the data were analyzed using ImageJ software (version 1.8.0, Md, uSa).

    Article Title: Real-time mass-resolved label-free single-molecule immunoassay
    Article Snippet: .. 1% casein solution (Cat: 37528) and phosphate-buffered saline (PBS, Cat: AM9625) solution were obtained from ThermoFisher Scientific. .. PBS and casein solution were filtered with a 0.22 μ m Millex-GP syringe filter (Millipore Sigma, Cat: SLGP033RS).

    Article Title: Combined TIRF and 3D Super-Resolution Microscopy for Nanoscopic Spatiotemporal Characterization of Adhesion Molecules on Microvilli
    Article Snippet: After washing off unbound protein A with HBSS, the chamber was incubated with a rhE-selectin (Sino Biological), which contained the fused C-terminal polyhistidine-tagged Fc region of human immunoglobulin G1 (IgG1) at the C terminus, at 4°C at concentrations of 0.2 μg ml −1 for 1 hour. .. The chamber was then washed with HBSS and blocked with 1% casein solution in phosphate-buffered saline (PBS; Thermo) for 30 min to 1 hour at room temperature. ..



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